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BL2011 Bioscience Practical Skills

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  School of Pharmacy and Biomedical SciencesUCLan Coursework Assessment Brief2021-22
Module Title: Bioscience Practical Skills Module Code: BL2011Level 5
  Lab Report – RESITThis assessment is worth 15% of the overall module mark
 
THE BRIEF/INSTRUCTIONS   You are required to write a 1500-word report about specific practical sessions/experiments.   The report must include each of the following parts (marking criteria & suggested word count for each section are shown in brackets):   General Introduction (10%, 300 words) – Here you should included a background to the AMPD1 enzyme based on the knowledge gathered from previous practicals 1-5.   Part A: Enzymatic activity of one natural and two cloned AMPD1 enzymes (25%, 350 words)   You will conduct an experiment (Practical 6 – Determination of the Activity of Human AMPD1 Enzyme). Following this session, you will be required to conduct another experiment (Practical 7 Determination of the activity of cloned and human AMPD1 enzymes) and propose a hypothesis about the activity of one natural and two cloned AMPD1 enzymes and design an experiment to test your hypothesis. You will need to perform analyses of AMPD1 enzymes from a variety of sources to determine the Km and Vmax.  Hypothesis and Aims of the experiment – state the aims that the experiment(s) set out to test and formulate a hypothesis • Materials and Methods – provide details of how the experiment was carried out and what materials were used. These details should have been recorded in your laboratory notebook. • Results – present data including a data analysis following the steps: observation, interpretation, and conclusion. NB: No statistical analysis is needed for this part. • Brief conclusion statements – a commentary on the results from the part A section     Part B: Yeast and Bacterial expression systems to produce AMPD1 enzyme (40%, 450 words) Similarto the statistics exercise during workshop, you will need to perform appropriate statistical test(s) to determine if there is any significant difference in the production yield of the enzyme from each organism. You will need to integrate these results into your laboratory report assignment. Use the information and guidance you have been given in the statistics lectures and workshops (including the model answers) to help you. You will be provided with expression data in excel to perform an appropriate statistical analysis using SPSS.   Ensure you are using the resit dataset PART B.   Experimental design: Experiments were carried out to compare the amount of AMPD1 enzyme which bacteria and yeast can produce (the ‘yield’). To do this, we created yeast and bacteria which were able to produce the enzyme. We then set up ten identical cultures of the bacteria and ten identical cultures of the yeast and grew them overnight. The next day we harvested the cells and collected the enzyme. We then calculated the concentration of extracted enzyme from each culture.  Hypothesis and Aims of the experiment – state the aims that the experiment(s) set out to test and formulate a hypothesis. • Materials and Methods – provide details of how the experiment was carried out and what materials were used. These details should have been recorded in your laboratory notebook. • Results – present data including every single step of statistical data analysis following describing the findings with a commentary on the results. An appropriate statistical analysis should be performed on additional data, which you will be provided with prior to starting the assignment • Brief conclusion statements – a commentary on the results from the part B section   Part C: Discussion and Conclusion (20%, 400 words) This section should highlight the main findings of both parts and discuss them in the context of other published studies. Based your analysis issued from both parts, you should conclude the report by discussing which enzyme you would select to carry out the industrial production in the biotech company.   References (5%) – in-text citations and bibliography presented in the Harvard style (does not contribute to word-count) which should only be presented in the introduction and discussion sections.   Report Format: The report should be written in an appropriate scientific style and language.   Additional guidance can be found in the “Preparation of a Laboratory Report” document, available on Blackboard.   This assignment will contribute to the assessment of the following learning outcomes for this module: 1. Discuss and apply the variety of techniques used to identify, isolate, visualize and investigate biological specimens and samples. 2. Formulate and test a hypothesis using appropriate experimental design 3. Apply techniques to explore biological and pharmacological activity in biological samples 5. Identify, select, and perform appropriate statistical tests to analyze a range of biological data types   You will be marked on: • Ability to write in an appropriate scientific style • Comprehension of the background to the experiment • Formulation of clear aims and hypothesis • Methods section with sufficient detail to allow others to reproduce the experiment • Presentation of results and summary of findings • Quality of figures and tables (if used) • Ability to select, perform and report appropriate statistical tests • Discussion of results in the context of the available published literature • Appreciation of limitations of the experiment • Range of references cited and style of referencing   What happens if you do not attend the practical classes associated with this assessment? If you have not attended the practical classes associated with this assessment, then you will then fail the assessment. You may be offered a resit opportunity. If you have not attended the practical classes assigned to the resit assessment, you will receive a mark of zero for this component. In cases where you had a legitimate reason not to attend the assigned practical, then you should apply for mitigating circumstances (MC) in the usual manner.
 
PREPARATION FOR THE ASSESSMENT Preparation for this assessment includes the lectures, workshops and Learning science simulations during weeks 5 – 13 to provide the theory underpinning the laboratory activities. These include all aspects of the molecular biology techniques and the statistical analysis required. A support session will take place for each group during the week 14 to review the statistical process and understand common errors to prepare the students for writing the assessment.LINK to the Reading List: https://eu02.alma.exlibrisgroup.com/leganto/readinglist/lists/4377825250003821
 
RELEASE DATES AND HAND IN DEADLINE Assessment Release date:   1/07/2022                     Assessment Deadline Date and time: 28/7/2022, 23:59pm                                 Please note that this is the final time you can submit – not the time to submit! Due to the extensive number of individual submissions, your feedback/feed forward and mark for this assessment will be provided respectively 15 and 31 working days after the submission date.
  SUBMISSION DETAILS   You should submit a word document to Turnitin before the deadline. Word count. The lab report should be 1500 words in length, which includes section headings but not references. You will be permitted plus or minus 10%. Information provided beyond this word count will not be marked.
 
HELP AND SUPPORT   Questions relating to the assessment should be posted on the BL2011 Bioscience Practical Teams site so that the respective staff member can answer specific questions and the entire group can benefit from the Q&A.Check all support materials provided in the BL2011 blackboard under the assignment tab.There will be one support session per group dedicated to understanding the assessment and using feedback to improve the final submission.For support with using library resources, please contact lsmith53@uclan.ac.uk or SubjectLibrarians@uclan.ac.uk. You will find links to lots of useful resources in the My Library tab on Blackboard.If you have not yet made the university aware of any disability, specific learning difficulty, long-term health, or mental health condition, please complete a Disclosure Form.  The Inclusive Support team will then contact to discuss reasonable adjustments and support relating to any disability.  For more information, visit the Inclusive Support site.To access mental health and wellbeing support, please complete our online referral form. Alternatively, you can email wellbeing@uclan.ac.uk, call 01772 893020 or visit our UCLan Wellbeing Service pages for more information.If you have any other query or require further support you can contact Student Support. Speak with us for advice on accessing all the University services as well as the Library services. Whatever your query, our expert staff will be able to help and support you. For more information , how to contact us and our opening hours visit Student Information and Support Centre.If you have any valid mitigating circumstances that mean you cannot meet an assessment submission deadline and you wish to request an extension, you will need to apply online prior to the deadline.
 
 
Disclaimer: The information provided in this assessment brief is correct at time of publication. In the unlikely event that any changes are deemed necessary, they will be communicated clearly via e-mail and a new version of this assessment brief will be circulated.Version: 1